A15785

antibody from Invitrogen Antibodies

Targeting: PDGFRA CD140a, GAS9, PDGFR2

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A15785

Provider

Invitrogen Antibodies

Product name

PDGFRA Monoclonal Antibody (16A1), PE

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Conjugate

Yellow dye

Isotype

IgG

Antibody clone number

16A1

Vial size

100 Tests

Storage

4° C

References

Submitted references

N-acetylglucosamine drives myelination by triggering oligodendrocyte precursor cell differentiation.

Sy M, Brandt AU, Lee SU, Newton BL, Pawling J, Golzar A, Rahman AMA, Yu Z, Cooper G, Scheel M, Paul F, Dennis JW, Demetriou M
The Journal of biological chemistry 2020 Dec 18;295(51):17413-17424

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 1. GlcNAc and N -glycan branching promotes oligodendrogenesis. A - D, flow cytometry of E12.5 NSCs from CD1 ( B and C ) or C57BL/6 ( D ) mice cultured in growth media (FGF + EGF) +- GlcNAc for 48 h. Cell surface binding levels of L-PHA and PDGFRalpha are measured as mean fluorescence intensity ( MFI ). E , flow cytometry and immunofluorescence microscopy of E12.5 NSCs in differentiation media (FGF + PDGF-AA) from Mgat5 +/+ , Mgat5 +/- , and Mgat5 -/- C57BL/6 mice. Data are three technical replicates per group ( A - E ), representative of 3 ( A - C ) or two ( D and E ) experiments. p -values are by one-way ANOVA with Sidak's multiple comparison test. All error bars are standard error. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Conjugate

Yellow dye