Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AP14398PU-N - Provider product page
- Provider
- OriGene
- Product name
- PDGF Receptor alpha (PDGFRA) rabbit polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- PDGF Receptor alpha (PDGFRA) rabbit polyclonal antibody, Aff - Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PDGFRA
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PDGFRA (arrow) using rabbit polyclonal PDGFRA Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PDGFRA gene (Lane 2).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- (LEFT)Immunofluorescence analysis of anti-PDGFRA polyclonal antibody in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI. (RIGHT)Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PDGFRA Antibody (Y754), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
- Validation comment
- IHC