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Proximity ligation assayAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Proximity ligation assay [1]
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- Product number
- H00005156-M02 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00005156-M02, RRID:AB_1112015
- Product name
- PDGFRA monoclonal antibody (M02), clone 4H1-1C9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full-length recombinant PDGFRA.
- Antigen sequence
LSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQY
PMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAH
TGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFV
PLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNS
EGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQ
TIPFNVYALKGTCIISFLL- Isotype
- IgG
- Antibody clone number
- 4H1-1C9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Dicer1 and miR-219 Are required for normal oligodendrocyte differentiation and myelination.
Dugas JC, Cuellar TL, Scholze A, Ason B, Ibrahim A, Emery B, Zamanian JL, Foo LC, McManus MT, Barres BA
Neuron 2010 Mar 11;65(5):597-611
Neuron 2010 Mar 11;65(5):597-611
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between CRK and PDGFRA. HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-PDGFRA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
