Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 323502 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#323502, RRID:AB_755996
- Product name
- Purified anti-human CD140a (PDGFR)
- Antibody type
- Monoclonal
- Antigen
- NIH 3T3 cells transfected with human PDGFRalpha
- Description
- The 16A1 monoclonal antibody recognizes human CD140a also known as the platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, and PDGFRα. CD140a is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140a contains three immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 123 kD. CD140a is widely expressed on a variety of mesenchymal-derived cells and has been implicated in the development of some tumors including basal cell carcinoma and gastric stromal cell tumors. Binding of A-chain containing PDGF molecules as well as protease-activated PDGF-C molecules can stimulate cell proliferation. CD140a has been shown to interact with a number of proteins including CRK, Grb2, Grb14, SHP2, and others as integrin β3, caveolin-1, and nexin sorting molecules. The PDGFRα is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. The 16A1 antibody has been shown to be useful for flow cytometric detection of CD140a.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C.
Submitted references Transcriptional profiles of CD133+ and CD133- glioblastoma-derived cancer stem cell lines suggest different cells of origin.
Lottaz C, Beier D, Meyer K, Kumar P, Hermann A, Schwarz J, Junker M, Oefner PJ, Bogdahn U, Wischhusen J, Spang R, Storch A, Beier CP
Cancer research 2010 Mar 1;70(5):2030-40
Cancer research 2010 Mar 1;70(5):2030-40
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human PDGFRA transfected cells stained with purified 16A1, followed by anti-mouse IgG FITC
- Conjugate
- Unconjugated