323503

antibody from BioLegend
Targeting: PDGFRA CD140a, GAS9, PDGFR2
Provider product page for 323503
Immunocytochemistry
Flow cytometry

Antibody data

Product number
323503 - Provider product page
Provider
BioLegend
Proper citation
BioLegend Cat#323503, RRID:AB_755997
Product name
Biotin anti-human CD140a (PDGFR‡)
Antibody type
Monoclonal
Antigen
NIH 3T3 cells transfected with human PDGFRalpha
Description
The 16A1 monoclonal antibody recognizes human CD140a also known as the platelet-derived growth factor receptor, alpha polypeptide, PDGFR2, and PDGFRα. CD140a is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The identity of the growth factor bound to the receptor determines whether the functional receptor is a homodimer or heterodimer composed of both PDGFR-α and -β. CD140a contains three immunoglobulin-like domains and a tyrosine kinase domain with a predicted molecular weight approximately 123 kD. CD140a is widely expressed on a variety of mesenchymal-derived cells and has been implicated in the development of some tumors including basal cell carcinoma and gastric stromal cell tumors. Binding of A-chain containing PDGF molecules as well as protease-activated PDGF-C molecules can stimulate cell proliferation. CD140a has been shown to interact with a number of proteins including CRK, Grb2, Grb14, SHP2, and others as integrin β3, caveolin-1, and nexin sorting molecules. The PDGFRα is heavily phosphorylated on numerous tyrosine residues through both autophosphorylation and ligand-dependent processes. The 16A1 antibody has been shown to be useful for flow cytometric detection of CD140a.
Reactivity
Human
Host
Mouse
Conjugate
Biotin
Isotype
IgG
Vial size
25 µg
Concentration
0.5 mg/ml
Storage
The antibody solution should be stored undiluted at 4°C. Do not freeze.
PDGFRA protein structure - 323503 shown in red.