GTX25452

antibody from GeneTex

Targeting: PDGFRA CD140a, GAS9, PDGFR2

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX25452

Provider

GeneTex

Proper citation

GeneTex Cat#GTX25452, RRID:AB_380534

Product name

PDGF Receptor alpha (phospho Tyr742) antibody

Antibody type

Polyclonal

Reactivity

Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Extracts prepared from NIH3T3 cells left unstimulated (1) and stimulated with PDGF (2-5) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4¢XC, then were incubated with 0.50 ?g/ml PDGFRalpha [pY742] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphotyrosine containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG alkaline phosphatase and bands were detected. The data show that only the peptide corresponding to PDGFRalpha [pY742] blocks the antibody signal, thereby demonstrating the specificity of the antibody.