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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF8520 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PSAP Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human PSAP in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC, as supplied. 1 month, 2 to 8 degreesC under sterile conditions after opening. 6 months, -20 to -70 degreesC under sterile conditions after opening.
Submitted references Characterization of lysosomal proteins Progranulin and Prosaposin and their interactions in Alzheimer's disease and aged brains: increased levels correlate with neuropathology.
Mendsaikhan A, Tooyama I, Bellier JP, Serrano GE, Sue LI, Lue LF, Beach TG, Walker DG
Acta neuropathologica communications 2019 Dec 21;7(1):215
Acta neuropathologica communications 2019 Dec 21;7(1):215
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human PSAP by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human PSAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8520) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for PSAP at approximately 60-80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
