Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
Reference
Comment
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- Product number
- 13358-1-AP - Provider product page
- Provider
- Proteintech Group
- Proper citation
- Proteintech Cat#13358-1-AP, RRID:AB_2269268
- Product name
- RAD17 antibody
- Antibody type
- Polyclonal
- Description
- RAD17 antibody (Cat. #13358-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IF, IHC, WB,ELISA.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 20ul, 150ul
Submitted references Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance.
Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway.
The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells.
Hunter JE, Campbell AE, Butterworth JA, Sellier H, Hannaway NL, Luli S, Floudas A, Kenneth NS, Moore AJ, Brownridge PJ, Thomas HD, Coxhead J, Taylor L, Leary P, Hasoon MSR, Knight AM, Garrett MD, Collins I, Eyers CE, Perkins ND
The Biochemical journal 2022 Oct 14;479(19):2087-2113
The Biochemical journal 2022 Oct 14;479(19):2087-2113
Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway.
Hunter JE, Campbell AE, Hannaway NL, Kerridge S, Luli S, Butterworth JA, Sellier H, Mukherjee R, Dhillon N, Sudhindar PD, Shukla R, Brownridge PJ, Bell HL, Coxhead J, Taylor L, Leary P, Hasoon MSR, Collins I, Garrett MD, Eyers CE, Perkins ND
The Biochemical journal 2022 Oct 14;479(19):2063-2086
The Biochemical journal 2022 Oct 14;479(19):2063-2086
The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells.
Schleicher EM, Dhoonmoon A, Jackson LM, Khatib JB, Nicolae CM, Moldovan GL
Oncogenesis 2022 Jun 18;11(1):33
Oncogenesis 2022 Jun 18;11(1):33
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Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 13358-1-AP(RAD17 antibody) at dilution of 1:400
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Hela cells, using RAD17 antibody 13358-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
- Sample type
- cell line
Supportive validation
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 13358-1-AP(RAD17 antibody) at dilution of 1:100 (under 10x lens)
- Sample type
- tissue
- Submitted by
- Proteintech Group (provider)
- Main image
- Experimental details
- Immunohistochemical of paraffin-embedded human breast cancer using 13358-1-AP(RAD17 antibody) at dilution of 1:100 (under 40x lens)
- Sample type
- tissue