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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [1]
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- Product number
- MAB1926 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Rad17 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Rad17 in Western blots.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Immunoprecipitation of Human Rad17. Rad17 was immunoprecipitated from 500 μg of K562 human chronic myelogenous leukemia cell line lysate following incubation with 2-3 µg Mouse Anti-Human Rad17 Monoclonal Antibody (Catalog # MAB1926) for 1 hour on ice. Rad17-antibody complexes were absorbed using goat anti-mouse IgG-agarose (Sigma). Immunoprecipitated Rad17 (left panel) was detected by Western blot using 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). For additional reference, Western blot (right panel) shows lysates of K562 cell line probed with 1 µg/mL Human/Rat Rad17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1926). View our recommended buffer recipes for immunoprecipitation.
