711120

antibody from Invitrogen Antibodies

Targeting: RAD17 CCYC, RAD17Sp, Rad24

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

711120

Provider

Invitrogen Antibodies

Product name

Phospho-RAD17 (Ser656) Recombinant Polyclonal Antibody (1HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

1HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with Etoposide (100 µM/mL for 2hr) (Lane 2), K-562 (Lane 3) and K-562 treated with Etoposide (100 µM/mL for 2hr) (Lane 4). The blots were probed with Anti-Rad17 (pS656) Recombinant Rabbit Polyclonal Antibody (Product # 711120, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 77 kDa band corresponding to YAP (pS127) was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).