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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- NBP2-75345 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Rad17 Antibody
- Antibody type
- Polyclonal
- Description
- Affinity purified.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4degreesC.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rad17 Antibody [Unconjugated] [NBP2-75345] - Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Detection by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Rad17 Antibody [Unconjugated] [NBP2-75345] - Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. For blotting immunoprecipitated Rad17, antibody was used at 1:100. Detection by chemiluminescence with an exposure time of 30 seconds.
