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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-85713 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-RAD17 (Ser656) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Keep as concentrated solution. Positive Control: 293T (100 uM etoposide treatment for 2 hrs). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.25 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-RAD17 (Ser656) in untreated (–) and treated (+) 293T whole cell extract using Phospho-RAD17 (Ser656) polyclonal antibody (Product # PA5-85713) using 50 µg of sample at a dilution of 1:500. Prior to incubation with primary antibody, the sample was separated on 7.5% SDS-PAGE.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Phospho-RAD17 (Ser656) Polyclonal Antibody (Product # PA5-85713). Untreated (–) and treated (+) 293T whole cell extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Phospho-RAD17 (Ser656) Polyclonal Antibody (Product # PA5-85713) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-RAD17 (Ser656) Polyclonal Antibody detects Rad17 (phospho Ser656) protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Rad17 (phospho Ser656) protein stained by Phospho-RAD17 (Ser656) Polyclonal Antibody (Product # PA5-85713) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-RAD17 (Ser656) Polyclonal Antibody detects Rad17 (phospho Ser656) protein at nucleus in human oral carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human oral carcinoma. Phospho-RAD17 (Ser656) Polyclonal Antibody (Product # PA5-85713) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Phospho-RAD17 (Ser656) Polyclonal Antibody detects Rad17 (phospho Ser656) protein at nucleus in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. Phospho-RAD17 (Ser656) Polyclonal Antibody (Product # PA5-85713) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
