HPA005448

antibody from Atlas Antibodies

Targeting: RAD17 CCYC, RAD17Sp, Rad24

Western blot (Enhanced validation)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

HPA005448

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005448, RRID:AB_1079738

Product name

Anti-RAD17

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human RAD17, Gene description: RAD17 homolog (S. pombe), Alternative Gene Names: CCYC, RAD17Sp, Rad24, Validated applications: IHC, ICC, WB, Uniprot ID: O75943, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells

Stadler C, Rexhepaj E, Singan V, Murphy R, Pepperkok R, Uhlén M, Simpson J, Lundberg E
Nature Methods 2013;10(4):315-323

---

Variance decomposition of protein profiles from antibody arrays using a longitudinal twin model

Kato B, Nicholson G, Neiman M, Rantalainen M, Holmes C, Barrett A, Uhlén M, Nilsson P, Spector T, Schwenk J
Proteome Science 2011;9(1):73

Western blot

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Recombinant expression validation

Main image

Experimental details

Western blot analysis in control (vector only transfected HEK293T lysate) and RAD17 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY403335).

Sample type

Human

Protocol

Protocol