Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27491 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HGS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: H1299, HeLa, mouse brain, rat brain. Predicted reactivity: Mouse (85%), Rat (82%), Dog (92%), Rhesus Monkey (98%), Bovine (89%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Host ESCRT factors are recruited during chikungunya virus infection and are required for the intracellular viral replication cycle.
Torii S, Orba Y, Sasaki M, Tabata K, Wada Y, Carr M, Hobson-Peters J, Hall RA, Takada A, Fukuhara T, Matsuura Y, Hall WW, Sawa H
The Journal of biological chemistry 2020 Jun 5;295(23):7941-7957
The Journal of biological chemistry 2020 Jun 5;295(23):7941-7957
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HGS using 50 µg rat brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HGS polyclonal antibody (Product # PA5-27491) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of HGS using 30 µg of A) H1299 and B) HeLa lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a HGS polyclonal antibody (Product # PA5-27491) at a dilution of 1:10,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HGS Polyclonal Antibody (Product # PA5-27491). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS Polyclonal Antibody (Product # PA5-27491) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using HGS Polyclonal Antibody (Product # PA5-27491). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with HGS Polyclonal Antibody (Product # PA5-27491) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of HGS in methanol-fixed A431 cells using a HGS polyclonal antibody (Product # PA5-27491) (Green) at a 1:200 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (red) at a 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using HGS (Product # PA5-27491) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
