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- References [3]
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- Immunohistochemistry [1]
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- Product number
- MAB25571 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Progranulin/PGRN Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Progranulin/PGRN in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human Progranulin is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Progranulin Decreases Susceptibility to Streptococcus pneumoniae in Influenza and Protects against Lethal Coinfection.
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Human tumors instigate granulin-expressing hematopoietic cells that promote malignancy by activating stromal fibroblasts in mice.
Wang X, Yuan J, Wang H, Gan N, Zhang Q, Liu B, Wang J, Shu Z, Rao L, Gou X, Zhang H, Yin Y, Zhang X
Journal of immunology (Baltimore, Md. : 1950) 2019 Oct 15;203(8):2171-2182
Journal of immunology (Baltimore, Md. : 1950) 2019 Oct 15;203(8):2171-2182
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Sinfield JK, Das A, O'Regan DJ, Ball SG, Porter KE, Turner NA
Biochemical and biophysical research communications 2013 Jan 4;430(1):419-24
Biochemical and biophysical research communications 2013 Jan 4;430(1):419-24
Human tumors instigate granulin-expressing hematopoietic cells that promote malignancy by activating stromal fibroblasts in mice.
Elkabets M, Gifford AM, Scheel C, Nilsson B, Reinhardt F, Bray MA, Carpenter AE, Jirström K, Magnusson K, Ebert BL, Pontén F, Weinberg RA, McAllister SS
The Journal of clinical investigation 2011 Feb;121(2):784-99
The Journal of clinical investigation 2011 Feb;121(2):784-99
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Progranulin in Mouse Kidney. Progranulin was detected in perfusion fixed frozen sections of mouse kidney using Rat Anti-Mouse Progranulin Monoclonal Antibody (Catalog # MAB25571) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
