Antibody data
- Antibody Data
- Antigen structure
- References [16]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AF2557 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Progranulin/PGRN Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Progranulin in direct ELISAs and Western blots. In Western blots, approximately 15% cross-reactivity with recombinant human Progranulin is observed.
- Reactivity
- Mouse
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P28798
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Genetic Regulation of Neuronal Progranulin Reveals a Critical Role for the Autophagy-Lysosome Pathway.
Microglia increases the proliferation of retinal precursor cells during postnatal development.
Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity.
Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle.
Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.
Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis.
Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum.
Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease.
Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice.
Lysosomal processing of progranulin.
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina.
Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells.
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Progranulin regulates neuronal outgrowth independent of sortilin.
Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins.
Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Elia LP, Mason AR, Alijagic A, Finkbeiner S
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Apr 24;39(17):3332-3344
The Journal of neuroscience : the official journal of the Society for Neuroscience 2019 Apr 24;39(17):3332-3344
Microglia increases the proliferation of retinal precursor cells during postnatal development.
Kuse Y, Ohuchi K, Nakamura S, Hara H, Shimazawa M
Molecular vision 2018;24:536-545
Molecular vision 2018;24:536-545
Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity.
Nicholson AM, Zhou X, Perkerson RB, Parsons TM, Chew J, Brooks M, DeJesus-Hernandez M, Finch NA, Matchett BJ, Kurti A, Jansen-West KR, Perkerson E, Daughrity L, Castanedes-Casey M, Rousseau L, Phillips V, Hu F, Gendron TF, Murray ME, Dickson DW, Fryer JD, Petrucelli L, Rademakers R
Acta neuropathologica communications 2018 May 31;6(1):42
Acta neuropathologica communications 2018 May 31;6(1):42
Progranulin deficiency leads to prolonged persistence of macrophages, accompanied with myofiber hypertrophy in regenerating muscle.
Sugihara H, Miyaji K, Yamanouchi K, Matsuwaki T, Nishihara M
The Journal of veterinary medical science 2018 Mar 2;80(2):346-353
The Journal of veterinary medical science 2018 Mar 2;80(2):346-353
Targeting Tyro3 ameliorates a model of PGRN-mutant FTLD-TDP via tau-mediated synaptic pathology.
Fujita K, Chen X, Homma H, Tagawa K, Amano M, Saito A, Imoto S, Akatsu H, Hashizume Y, Kaibuchi K, Miyano S, Okazawa H
Nature communications 2018 Jan 30;9(1):433
Nature communications 2018 Jan 30;9(1):433
Progranulin Gene Therapy Improves Lysosomal Dysfunction and Microglial Pathology Associated with Frontotemporal Dementia and Neuronal Ceroid Lipofuscinosis.
Arrant AE, Onyilo VC, Unger DE, Roberson ED
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 28;38(9):2341-2358
The Journal of neuroscience : the official journal of the Society for Neuroscience 2018 Feb 28;38(9):2341-2358
Retrograde Signaling from Progranulin to Sort1 Counteracts Synapse Elimination in the Developing Cerebellum.
Uesaka N, Abe M, Konno K, Yamazaki M, Sakoori K, Watanabe T, Kao TH, Mikuni T, Watanabe M, Sakimura K, Kano M
Neuron 2018 Feb 21;97(4):796-805.e5
Neuron 2018 Feb 21;97(4):796-805.e5
Progranulin gene delivery reduces plaque burden and synaptic atrophy in a mouse model of Alzheimer's disease.
Van Kampen JM, Kay DG
PloS one 2017;12(8):e0182896
PloS one 2017;12(8):e0182896
Conditional loss of progranulin in neurons is not sufficient to cause neuronal ceroid lipofuscinosis-like neuropathology in mice.
Petkau TL, Blanco J, Leavitt BR
Neurobiology of disease 2017 Oct;106:14-22
Neurobiology of disease 2017 Oct;106:14-22
Lysosomal processing of progranulin.
Zhou X, Paushter DH, Feng T, Sun L, Reinheckel T, Hu F
Molecular neurodegeneration 2017 Aug 23;12(1):62
Molecular neurodegeneration 2017 Aug 23;12(1):62
Progranulin promotes the retinal precursor cell proliferation and the photoreceptor differentiation in the mouse retina.
Kuse Y, Tsuruma K, Sugitani S, Izawa H, Ohno Y, Shimazawa M, Hara H
Scientific reports 2016 Mar 31;6:23811
Scientific reports 2016 Mar 31;6:23811
Effects of hypoxia on progranulin expression in HT22 mouse hippocampal cells.
Luo L, Lü L, Lu Y, Zhang L, Li B, Guo K, Chen L, Wang Y, Shao Y, Xu J
Molecular medicine reports 2014 May;9(5):1675-80
Molecular medicine reports 2014 May;9(5):1675-80
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Sinfield JK, Das A, O'Regan DJ, Ball SG, Porter KE, Turner NA
Biochemical and biophysical research communications 2013 Jan 4;430(1):419-24
Biochemical and biophysical research communications 2013 Jan 4;430(1):419-24
Progranulin regulates neuronal outgrowth independent of sortilin.
Gass J, Lee WC, Cook C, Finch N, Stetler C, Jansen-West K, Lewis J, Link CD, Rademakers R, Nykjær A, Petrucelli L
Molecular neurodegeneration 2012 Jul 10;7:33
Molecular neurodegeneration 2012 Jul 10;7:33
Progranulin, a glycoprotein deficient in frontotemporal dementia, is a novel substrate of several protein disulfide isomerase family proteins.
Almeida S, Zhou L, Gao FB
PloS one 2011;6(10):e26454
PloS one 2011;6(10):e26454
Progranulin expression correlates with dense-core amyloid plaque burden in Alzheimer disease mouse models.
Pereson S, Wils H, Kleinberger G, McGowan E, Vandewoestyne M, Van Broeck B, Joris G, Cuijt I, Deforce D, Hutton M, Van Broeckhoven C, Kumar-Singh S
The Journal of pathology 2009 Oct;219(2):173-81
The Journal of pathology 2009 Oct;219(2):173-81
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of mouse uterus tissue and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Progranulin/PGRN at approximately 85-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Progranulin/PGRN in Mouse Splencytes. Progranulin/PGRN was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse Progranulin/PGRN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Progranulin in Mouse Kidney. Progranulin was detected in immersion fixed frozen sections of mouse kidney using Sheep Anti-Mouse Progranulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2557) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.