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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MAB24201-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Progranulin/PGRN Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Progranulin/PGRN in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P28799- Isotype
- IgG
- Antibody clone number
- 296605
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse Progranulin/PGRN by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and mouse liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Progranulin/PGRN Polyclonal Antibody (Catalog # MAB24201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Progranulin/PGRN at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. For Western blotting, Catalog # AF2420 is recommended.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Progranulin/PGRN in Human Breast Cancer Tissue. Progranulin/PGRN was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Progranulin/PGRN Polyclonal Antibody (Catalog # MAB24201) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
