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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- 710191 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Granulins Recombinant Polyclonal Antibody (2HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- 2HCLC
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Granulins in MCF-7 whole cell extracts using a Granulins Recombinant Rabbit Polyclonal Antibody (Product # 710191) at a dilution of 2 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~64kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Granulins in HEK293 cells using a Granulins Recombinant Rabbit Polyclonal Antibody (Product # 710191) followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (green) (Image A). Nuclei were stained using DAPI (Image B) and a composite image showing actin stained using Alexa Fluor 594 phalloidin (red). Image D is a composite image showing membrane localization.
