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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AF4548 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Daxx Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects endogenous human Daxx in Western blots. Detection of both reported Daxx isoforms is expected based on immunogen sequence.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Human Daxx by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Ramos human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human Daxx Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4548) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Daxx at approximately 115 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
