Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB0448 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0448, RRID:AB_982492
- Product name
- DAXX (phospho S213) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of DAXX.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Daxx mediates the small ubiquitin-like modifier-dependent transcriptional repression of Smad4.
Interaction between the human cytomegalovirus UL82 gene product (pp71) and hDaxx regulates immediate-early gene expression and viral replication.
The cellular protein daxx interacts with avian sarcoma virus integrase and viral DNA to repress viral transcription.
Chang CC, Lin DY, Fang HI, Chen RH, Shih HM
The Journal of biological chemistry 2005 Mar 18;280(11):10164-73
The Journal of biological chemistry 2005 Mar 18;280(11):10164-73
Interaction between the human cytomegalovirus UL82 gene product (pp71) and hDaxx regulates immediate-early gene expression and viral replication.
Cantrell SR, Bresnahan WA
Journal of virology 2005 Jun;79(12):7792-802
Journal of virology 2005 Jun;79(12):7792-802
The cellular protein daxx interacts with avian sarcoma virus integrase and viral DNA to repress viral transcription.
Greger JG, Katz RA, Ishov AM, Maul GG, Skalka AM
Journal of virology 2005 Apr;79(8):4610-8
Journal of virology 2005 Apr;79(8):4610-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The DAXX (phospho S213) polyclonal antibody (Cat # PAB0448) is used in Western blot to detect Phospho-DAXX-S213 in mouse thymus tissue lysate
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with DAXX (phospho S213) polyclonal antibody (Cat # PAB0448) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)