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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [5]
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- Product number
- MA1-19296 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DAXX Monoclonal Antibody (DAXX-01)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- This antibody recognizes Daxx, a cytoplasmic death domain containing protein mainly expressed in fetal and adult human and mouse tissue.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- DAXX-01
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DAXX Monoclonal Antibody (DAXX-01) (Product # MA1-19296) and a 110kDa band corresponding to DAXX was observed across all cell lines tested. Whole Cell Extract-WCL (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), K-562 (Lane 3), MCF7 (Lane 4), U-2 OS (Lane 5) and SH-SY5Y (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).Two uncharacterized bands were also observed at ~26kDa and 48kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DAXX using a monoclonal antibody (Product # MA1-19296).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DAXX using a monoclonal antibody (Product # MA1-19296).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DAXX using a monoclonal antibody (Product # MA1-19296).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DAXX using a monoclonal antibody (Product # MA1-19296).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01) Monoclonal antobody (Product # MA1-19296), nuclei were stained with DAPI (blue). A - nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxx. B - HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm.
