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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-J22 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-J22, RRID:AB_2614382
- Product name
- Anti-HDAC7 (RABBIT) Antibody - 600-401-J22
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-HDAC7 antibody. Lane 1: mouse brain homogenate. Lane 2: mouse brain homogenate blocked with peptide. Load: 4 µg per lane. Primary antibody: HDAC7 antibody at 1µg/mL for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~103kDa/ ~103, 70, and 40kDa for HDAC7. Other band(s): HDAC7 isoforms ~70 and 40kDa.
- Validation comment
- Western Blot
