Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB3541-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BMP-7 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human BMP-7 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human (rh) BMP-6 is observed and no cross-reactivity with rhBMP-2, -3, -4, -5, or -8 is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P18075
- Isotype
- IgG
- Antibody clone number
- 164311
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening.
Submitted references Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer.
Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade.
A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation.
Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
Blanchette-Farra N, Kita D, Konstorum A, Tesfay L, Lemler D, Hegde P, Claffey KP, Torti FM, Torti SV
Oncogene 2018 Jul;37(29):4013-4032
Oncogene 2018 Jul;37(29):4013-4032
Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade.
Du Y, Xiao Q, Yip HK
Investigative ophthalmology & visual science 2010 Jul;51(7):3764-73
Investigative ophthalmology & visual science 2010 Jul;51(7):3764-73
A new model for growth factor activation: type II receptors compete with the prodomain for BMP-7.
Sengle G, Ono RN, Lyons KM, Bächinger HP, Sakai LY
Journal of molecular biology 2008 Sep 12;381(4):1025-39
Journal of molecular biology 2008 Sep 12;381(4):1025-39
Aggressive melanoma cells escape from BMP7-mediated autocrine growth inhibition through coordinated Noggin upregulation.
Hsu MY, Rovinsky SA, Lai CY, Qasem S, Liu X, How J, Engelhardt JF, Murphy GF
Laboratory investigation; a journal of technical methods and pathology 2008 Aug;88(8):842-55
Laboratory investigation; a journal of technical methods and pathology 2008 Aug;88(8):842-55
Combinatorial gene therapy for bone regeneration: cooperative interactions between adenovirus vectors expressing bone morphogenetic proteins 2, 4, and 7.
Zhao M, Zhao Z, Koh JT, Jin T, Franceschi RT
Journal of cellular biochemistry 2005 May 1;95(1):1-16
Journal of cellular biochemistry 2005 May 1;95(1):1-16
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BMP-7 in Human Kidney. BMP-7 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human BMP-7 Monoclonal Antibody (Catalog # MAB3541) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP-7 and Neutralization by Human BMP-7 Antibody. Recombinant Human BMP-7 (Catalog # 354-BP) induces alkaline phosphatase production in the the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-7 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human BMP-7 Monoclonal Antibody (Catalog # MAB3541). The ND50 is typically 1.5-6.0 µg/mL in the presence of L-ascorbic acid (50 µg/mL).