H00000655-M03

antibody from Abnova Corporation

Targeting: BMP7 OP-1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000655-M03

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000655-M03, RRID:AB_509355

Product name

BMP7 monoclonal antibody (M03), clone S52

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant BMP7.

Antigen sequence

MHVRSLRAAAPHSFVALWAPLFLLRSALADFSLDN
EVHSSFIHRRLRSQERREMQREILSILGLPHRPRP
HLQGKHNSAPMFMLDLYNAMAVEEGGGPGGQGFSY
PYKAVFSTQGPPLASLQDSHFLTDADMVMSFVNLV
EHDKEFFHPRYHHREFRFDLSKIPEGEAVTAAEFR
IYKDYIRERFDNETFRISVYQVLQEHLGRESDLFL
LDSRTLWASEEGWLVFDITATSNHWVVNPRHNLGL
QLSVETLDGQSINPKLAGLIGRHGPQNKQPFMVAF
FKATEVHFRSIRSTGSKQRSQNRSKTPKNQEALRM
ANVAENSSSDQRQACKKHELYVSFRDLGWQDWIIA
PEGYAAYYCEGECAFPLNSYMNATNHAIVQTLVHF
INPETVPKPCCAPTQLNAISVLYFDDSSNVILKKY
RNMVVRACGCH


Isotype

IgG

Antibody clone number

S52

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in A-549 ( Cat # L025V1 ).

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BMP7 expression in transfected 293T cell line by BMP7 monoclonal antibody (M03), clone S52.Lane 1: BMP7 transfected lysate(49.313 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BMP7 is 10 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol