PA5-11720

antibody from Invitrogen Antibodies

Targeting: BMP7 OP-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-11720

Provider

Invitrogen Antibodies

Product name

BMP-7 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with mouse based on sequence homology.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

1.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Bmp7 (arrow) using a Bmp7 polyclonal antibody (Product # PA5-11720) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the Bmp7 gene (Lane 2).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-BMP-7 Polyclonal Antibody (Product # PA5-11720) and a 50 kDa band corresponding to Bmp7 was observed across cell lines tested. The expression was found to be low in HeLa, PC-3, HCT 116, BJ and Hep G2 as reported. Whole cell extracts (30 µg lysate) of MCF7 (Lane 1), T-47D (Lane 2), HeLa (Lane 3), PC-3 (Lane 4), HCT 116 (Lane 5), BJ (Lane 6) and Hep G2 (Lane 7) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580). Uncharacterized bands were observed around 30 and 55 kDa.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis using a Bmp7 polyclonal antibody (Product # PA5-11720) in HeLa cells. 0.025 mg/mL primary antibody was followed by fluor-conjugated donkey anti-rabbit lgG (H+L) (orange). Blue counterstaining is DAPI.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Bmp7 was performed using 70% confluent log phase MCF7 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with BMP-7 Polyclonal Antibody (Product # PA5-11720) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear and cytoplasmic localization of BMP7 in MCF7. Panel e represents HeLa cells with lower expression of BMP7. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cancer tissue using a Bmp7 polyclonal antibody (Product # PA5-11720), followed by HRP-conjugated secondary antibody and DAB staining.