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- References [3]
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- Product number
- 500-P198-100UG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BMP-7 Polyclonal Antibody, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MANVAENSSS DQRQACKKHE LYVSFRDLGW QDWIIAPEGY AAYYCEGECA FPLNSYMNAT NHAIVQTLVH FINPETVPKP CCAPTQLNAI SVLYFDDSSN VILKKYRNMV VRACGCH. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human BMP-7. Anti-Human BMP-7-specific antibody was purified by affinity chromatography employing an immobilized Human BMP-7 matrix. Sandwich ELISA: To detect Human BMP-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human BMP-7 (500-P198Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BMP-7. Western Blot: To detect Human BMP-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BMP-7 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 100 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references A new MMP-mediated prodomain cleavage mechanism to activate bone morphogenetic proteins from the extracellular matrix.
Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
Bmp7 regulates the survival, proliferation, and neurogenic properties of neural progenitor cells during corticogenesis in the mouse.
Furlan AG, Spanou CES, Godwin ARF, Wohl AP, Zimmermann LA, Imhof T, Koch M, Baldock C, Sengle G
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Mar;35(3):e21353
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021 Mar;35(3):e21353
Extracellular Regulation of Bone Morphogenetic Protein Activity by the Microfibril Component Fibrillin-1.
Wohl AP, Troilo H, Collins RF, Baldock C, Sengle G
The Journal of biological chemistry 2016 Jun 10;291(24):12732-12746
The Journal of biological chemistry 2016 Jun 10;291(24):12732-12746
Bmp7 regulates the survival, proliferation, and neurogenic properties of neural progenitor cells during corticogenesis in the mouse.
Segklia A, Seuntjens E, Elkouris M, Tsalavos S, Stappers E, Mitsiadis TA, Huylebroeck D, Remboutsika E, Graf D
PloS one 2012;7(3):e34088
PloS one 2012;7(3):e34088
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Sandwich ELISA: To detect Human BMP-7 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of BMP-7 Polyclonal Antibody (Product # 500-P198-1MG) is required. This antigen affinity purified antibody, in conjunction with PeproTech BMP-7 Polyclonal Antibody, Biotin (Product # 500-P198BT-1MG) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human BMP-7.
