Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 720363 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BDKRB2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.
- Concentration
- 0.5 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK 293 cells were transiently transfected with Bradykinin receptor B2/BDKRB2 or Formyl peptide receptor 2/(FPR2. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-Bradykinin receptor B2/BDKRB2) Rabbit Polyclonal Antibody (Product # 720363 at a concentration of 2 µg/mL). Bands from 60-100 kDa corresponding to Bradykinin receptor B2/BDKRB2 was observed.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HEK 293 cells were transiently transfected with Bradykinin receptor B2/BDKRB2 or Formyl peptide receptor 2/(FPR2. After 48 h, cells were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with Anti-Bradykinin receptor B2/BDKRB2) Rabbit Polyclonal Antibody (Product # 720363 at a concentration of 2 µg/mL). Bands from 60-100 kDa corresponding to Bradykinin receptor B2/BDKRB2 was observed.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sections of human pancreas were dewaxed, microwaved in citric acid and incubated with Anti-Bradykinin receptor B2/BDKRB2 Rabbit Polyclonal Antibody (Product # 720363 at a concentration of 2 µg/mL). Sections were then sequentially treated with biotinylated anti-rabbit IgG and AB solution. Sections were then developed in AEC and lightly counterstained with hematoxylin. Inset, for adsorption controls the antibody was incubated with 10 µg/mL of the peptide used for immunizations (+ Peptide). Note, staining of pancreatic islets is completely neutralized by preincubation of the antibody with its immunizing peptide.