Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB9434-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Bradykinin RB2/BDKRB2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human RB2/BDKRB2 in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P30411
- Isotype
- IgG
- Antibody clone number
- 471902
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Bradykinin RB2/BDKRB2 by Western Blot. Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human Bradykinin RB2/BDKRB2. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB9434) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Bradykinin RB2/BDKRB2 at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Bradykinin RB2/BDKRB2 in Human Liver Cancer Tissue. Bradykinin RB2/BDKRB2 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB9434) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Bradykinin RB2/BDKRB2 in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP by Flow Cytometry HEK293 human cell line transfected with (A) Human Bradykinin RB2/BDKRB2 or (B) irrelevant protein, and eGFP was stained with Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB9434) followed by APC-conjugated Goat anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on Mouse IgG1 Isotype Control Antibody (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.