Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- MAB47251-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Bradykinin RB2/BDKRB2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Bradykinin RB2/BDKRB2 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P30411
- Isotype
- IgG
- Antibody clone number
- 471916
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Bradykinin RB2/BDKRB2 in Human Stomach. Bradykinin RB2/BDKRB2 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Bradykinin RB2/BDKRB2 in HEK293 Human Cell Line Transfected with Human Bradykinin RB2/BDKRB2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human Bradykinin RB2/BDKRB2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Bradykinin RB2/BDKRB2 Monoclonal Antibody (Catalog # MAB47251) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.