Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [3]
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Validation data
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- Product number
- ADI-905-747-100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ADI-905-747-100, RRID:AB_2038868
- Product name
- Bradykinin receptor B2, pAb
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide affinity purified. Liquid. In PBS containing 50% glycerol and 0.01% sodium azide.
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Supportive validation
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- Enzo Life Sciences (provider)
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- Experimental details
- Western blot analysis: MW marker (1) and 20µg rat brain extract (2) probed with Bradykinin Receptor (B2) pAb at 2.2µg/ml.
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- Enzo Life Sciences (provider)
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- Experimental details
- Immunohistochemistry analysis: Mouse brain fixed with 4% paraformaldehyde, stained with Bradykinin Receptor (B2) pAb at 1:1000, and visualized at 40x.
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- Enzo Life Sciences (provider)
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- Experimental details
- Membrane ELISA: Lewis rat heart membranes (5µg/well) were treated with 1 µM concentrations of agonist (BK) and probed with Bradykinin Receptor (B2) pAb (1:250 to 1:2000 of a 1µg/µL stock solution) by ELISA. Data from vehicle treated cells were taken as 100%. Results are the mean ± SEM (n=3).