TA324263

antibody from OriGene

Targeting: MCM3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA324263

Provider

OriGene

Product name

Anti-MCM3 Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-MCM3 Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

MCM3

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

0.8mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 91 kDa . Positive control: K562, Jurkat, hela and 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: K562 cell lysate Lane2: Jurkat cell lysate Lane3: Hela cell lysate Lane4: 293T cell lysate Lysates: 40 ug per lane Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human lung cancer tissue using MCM3 antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200)

Validation comment

IHC