Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-29230 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using MCM3 Polyclonal Antibody (Product # PA5-29230). Sample (30 µg of whole cell lysate). Lane A: 293T . 7.5% SDS PAGE. MCM3 Polyclonal Antibody (Product # PA5-29230) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-MCM3 Polyclonal Antibody (Product # PA5-29230) and a 100 kDa band corresponding to MCM3 was observed across all cell lines. Expression of MCM3 was observed to be downregulated upon treatment of LNCaP cells with Trichostatin A (100 ng/mL for 24 hours) (DOI: 10.1158/0008-5472.CAN-09-4176). Modified whole cell extracts (1% SDS) (50 µg lysate) of K-562 (Lane 1), MOLT-4 (Lane 2), Raji (Lane 3), SW480 (Lane 4), A549 (Lane 5), MCF7 (Lane 6), Jurkat (Lane 7), LNCaP (Lane 8) and LNCaP treated with Trichostatin A (100 ng/mL for 24 hours) (Lane 9) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Reagent Kit (Product # WP20005). An uncharacterized band (*) was observed at ~62 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- MCM3 Polyclonal Antibody detects MCM3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MCM3 protein stained by MCM3 Polyclonal Antibody (Product # PA5-29230) diluted at 1:500. Blue: Hoechst 33343 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 Xenograft, using MCM3 (Product # PA5-29230) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.