Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 358506 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#358506, RRID:AB_2562472
- Product name
- APC anti-human/mouse Bcl-6
- Antibody type
- Monoclonal
- Antigen
- Sequence 226-398 of murine BLC6 fused with GST
- Description
- B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer, member of the BTB-POZ zinc finger family. It contains 1 BTB (POZ) domain and 6 C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor, master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switch and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.
- Reactivity
- Human, Mouse
- Host
- Rat
- Conjugate
- Red dye
- Antibody clone number
- 7D1
- Vial size
- 100 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- 13-day immunized C57BL/6 mouse lymph node cells were surface stained with B220 Brilliant Violet 421™ and IgD PerCP/Cy5.5, then fixed and permeabilized with Nuclear Factor Fixation and Permeabilization Buffer Set. Cells were then stained with Bcl-6 (clone 7D1) APC (top) or rat IgG2a, κ APC isotype control (bottom). Data shown was gated on B220-positive cells.
- Conjugate
- Red dye