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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-31369 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal Bcl-6 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Bcl6 Antibody [NBP2-31369] - WB detection of B-cell lymphoma 6 / Bcl6 protein in (A) lysate of Ramos /human Burkitt's lymphoma cell line and (B) partial recombinant protein using Bcl6 antibody at a concentration of: 2ug/ml for lysate, and 0.5ug/ml for recombinant protein. In Ramos cells, this antibody detected a specific band below ~80 kDa (predicted size 78.9 kDa) and two additional bands ~60kDa/35kDa which are potentially the polyubiquitinization degradation fragments of this protein.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Bcl6 Antibody [NBP2-31369] - IHC analysis of formalin-fixed paraffin-embedded tissue section of human esophageal squamous cell carcinoma (SCC) using Bcl6 antibody at a concentration of 5 ug/ml. Nuclear as well as cytoplasmic immunoreactivity was observed for Bcl6 protein in SCC cells whereas the tumor stroma cells did not develop DAB staining. [40X Magnification]
