648305

antibody from BioLegend

Targeting: BCL6 BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

648305

Provider

BioLegend

Proper citation

BioLegend Cat#648305, RRID:AB_2565298

Product name

Alexa Fluor® 647 anti-mouse/human Bcl-6

Antibody type

Monoclonal

Antigen

Plasmid vector containing cDNA of the Bcl-6 gene

Description

B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer and member of the BTB-POZ zinc finger family.  It consists of one BTB (POZ) domain and six C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor as well as a master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switching and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to T_FH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Red dye

Antibody clone number

IG191E/A8

Vial size

25 tests

Storage

2°C-8°C

Handling

Ambient RT

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

C57BL/6 lymph node cells were immunized with CFA for 12 days, fixed, and permeabilized with BioLegend Nuclear Factor Fixation and Permeabilization Buffer Set, then stained with CD19 FITC, IgD PerCP/Cy5.5, and Bcl-6 (clone IG191E/A8) Alexa Fluor® 647 (top) or mouse IgG1, κ Alexa Fluor® 647 isotype control (bottom). Dot plots are gated on CD19⁺ lymphocytes.

Conjugate

Red dye