648308

antibody from BioLegend

Targeting: BCL6 BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

648308

Provider

BioLegend

Proper citation

BioLegend Cat#648308, RRID:AB_2629615

Product name

Alexa Fluor® 594 anti-mouse/human Bcl-6

Antibody type

Monoclonal

Antigen

Plasmid vector containing cDNA of the Bcl-6 gene

Reactivity

Human, Mouse

Host

Mouse

Conjugate

Yellow dye

Antibody clone number

IG191E/A8

Vial size

100 µg

Concentration

0.5 μ/ml

Storage

2°C-8°C

Handling

Ambient RT

Immunohistochemistry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% Gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of anti-mouse/human Bcl-6 (clone IG191E/A8) Alexa Fluor® 594 (red) and anti-human mac-2 (clone M3/38) Alexa Fluor® 674 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.

Conjugate

Yellow dye