Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- 648308 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#648308, RRID:AB_2629615
- Product name
- Alexa Fluor® 594 anti-mouse/human Bcl-6
- Antibody type
- Monoclonal
- Antigen
- Plasmid vector containing cDNA of the Bcl-6 gene
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- IG191E/A8
- Vial size
- 100 µg
- Concentration
- 0.5 μ/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human paraffin-embedded tonsil tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with Tris-Buffered Saline 20X (1.0M, pH7.4) at 95°C for 40 minutes. Tissue was washed with PBS/ 0.05% Tween20 twice for five minutes and blocked with 5% FBS and 0.2% Gelatin for 30 minutes. Then, the tissue was stained with 5 µg/ml of anti-mouse/human Bcl-6 (clone IG191E/A8) Alexa Fluor® 594 (red) and anti-human mac-2 (clone M3/38) Alexa Fluor® 674 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
- Conjugate
- Yellow dye