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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- 17-9880-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCL6 Monoclonal Antibody (BCL-UP), APC, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody BCL-UP recognizes Bcl-6, also known as B-cell lymphoma 6,which is a member of the POZ family of transcriptional repressors. Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79 kDa protein is essential for germinal center B cell proliferation and survival. During B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1. Applications Reported: This BCL-UP antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BCL-UP antibody has been pre-titrated and tested in human tonsil cells by intracellular staining with the Foxp3 Fixation/Permabilization Buffers (Product # 00-5521-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BCL-UP
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Circulating CXCR3(+) Tfh cells positively correlate with neutralizing antibody responses in HCV-infected patients.
Follicular regulatory T cells expressing Foxp3 and Bcl-6 suppress germinal center reactions.
Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.
BCL6 is critical for the development of a diverse primary B cell repertoire.
Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation.
Zhang J, Liu W, Wen B, Xie T, Tang P, Hu Y, Huang L, Jin K, Zhang P, Liu Z, Niu L, Qu X
Scientific reports 2019 Jul 12;9(1):10090
Scientific reports 2019 Jul 12;9(1):10090
Follicular regulatory T cells expressing Foxp3 and Bcl-6 suppress germinal center reactions.
Chung Y, Tanaka S, Chu F, Nurieva RI, Martinez GJ, Rawal S, Wang YH, Lim H, Reynolds JM, Zhou XH, Fan HM, Liu ZM, Neelapu SS, Dong C
Nature medicine 2011 Jul 24;17(8):983-8
Nature medicine 2011 Jul 24;17(8):983-8
Human tonsil B-cell lymphoma 6 (BCL6)-expressing CD4+ T-cell subset specialized for B-cell help outside germinal centers.
Bentebibel SE, Schmitt N, Banchereau J, Ueno H
Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 16;108(33):E488-97
Proceedings of the National Academy of Sciences of the United States of America 2011 Aug 16;108(33):E488-97
BCL6 is critical for the development of a diverse primary B cell repertoire.
Duy C, Yu JJ, Nahar R, Swaminathan S, Kweon SM, Polo JM, Valls E, Klemm L, Shojaee S, Cerchietti L, Schuh W, Jäck HM, Hurtz C, Ramezani-Rad P, Herzog S, Jumaa H, Koeffler HP, de Alborán IM, Melnick AM, Ye BH, Müschen M
The Journal of experimental medicine 2010 Jun 7;207(6):1209-21
The Journal of experimental medicine 2010 Jun 7;207(6):1209-21
Bcl6 and Blimp-1 are reciprocal and antagonistic regulators of T follicular helper cell differentiation.
Johnston RJ, Poholek AC, DiToro D, Yusuf I, Eto D, Barnett B, Dent AL, Craft J, Crotty S
Science (New York, N.Y.) 2009 Aug 21;325(5943):1006-10
Science (New York, N.Y.) 2009 Aug 21;325(5943):1006-10
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of human tonsil cells with Anti-Human CD4 eFluor® 450, CD19 PE, CD279 (PD-1) PE-Cy7, CD278 (ICOS) PerCP-eFluor® 710, and CD45RO FITC antibodies followed by intracellular staining with Anti-Human Bcl-6 APC antibody using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. The histogram (right) demonstrates Bcl-6 staining of CD4+CD45RO+CD278highCD279high (purple histogram) and CD4+CD45RO+CD278lowCD279low (blue histogram) cells. Cell populations were gated as indicated on the contour plot (left). Data courtesy of Danelle Eto (Laboratory of Shane Crotty).
