Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- 46-9880-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-BCL6 Monoclonal Antibody (BCL-UP), PerCP-eFluor 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody BCL-UP recognizes Bcl-6, also known as B-cell lymphoma 6,which is a member of the POZ family of transcriptional repressors. Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79 kDa protein is essential for germinal center B cell proliferation and survival. During B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1. Applications Reported: This BCL-UP antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This BCL-UP antibody has been pre-titrated and tested by intracellular flow cytometric analysis of human tonsil cells using the Foxp3 Fixation/Permabilization Buffers (cat. 00-5521) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative. Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BCL-UP
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Circulating CXCR5+PD-1+ response predicts influenza vaccine antibody responses in young adults but not elderly adults.
Herati RS, Reuter MA, Dolfi DV, Mansfield KD, Aung H, Badwan OZ, Kurupati RK, Kannan S, Ertl H, Schmader KE, Betts MR, Canaday DH, Wherry EJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3528-37
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3528-37
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of human tonsil cells with the Anti-Human CD4 eFluor® 450, CD19 APC-eFluor® 780, CD279 (PD-1) PE, CD278 (ICOS) PE-Cy7, and CD45RO FITC antibodies followed by intracellularstaining with Anti-Human Bcl-6 PerCP-eFluor® 710 antibody using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. The histogram (right) demonstrates Bcl-6 staining of CD4+CD45RO+CD278highCD279high (purple histogram) and CD4+CD45RO+CD278lowCD279low (blue histogram) cells. Cell populations were gated as indicated on the contour plot (left). Data courtesy of Danelle Eto (Laboratory of Shane Crotty).