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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- 46-9880-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BCL6 Monoclonal Antibody (BCL-UP), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody BCL-UP recognizes Bcl-6, also known as B-cell lymphoma 6,which is a member of the POZ family of transcriptional repressors. Bcl-6 is expressed most abundantly in germinal center B cells, as well as in differentiating and activated B cells. This 79 kDa protein is essential for germinal center B cell proliferation and survival. During B cell development, Bcl-6 plays an important role in promoting pre-B cell survival to enable the generation of a diverse B cell repertoire. Moreover, Bcl-6 is expressed constitutively in diffuse large B cell lymphomas (DLBCLs), Burkitt's lymphoma, and non-Hodgkin's lymphoma. Reports also suggest expression of Bcl-6 in CD4+ T follicular helper cells and memory T cells. Bcl-6 has been reported to be antagonized by Blimp-1.
- Antibody clone number
- BCL-UP
- Concentration
- 5 µL/Test
Submitted references Circulating CXCR5+PD-1+ response predicts influenza vaccine antibody responses in young adults but not elderly adults.
Herati RS, Reuter MA, Dolfi DV, Mansfield KD, Aung H, Badwan OZ, Kurupati RK, Kannan S, Ertl H, Schmader KE, Betts MR, Canaday DH, Wherry EJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3528-37
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3528-37
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Surface staining of human tonsil cells with the Anti-Human CD4 eFluor® 450, CD19 APC-eFluor® 780, CD279 (PD-1) PE, CD278 (ICOS) PE-Cy7, and CD45RO FITC antibodies followed by intracellularstaining with Anti-Human Bcl-6 PerCP-eFluor® 710 antibody using the Foxp3 Fixation/Permeabilization Buffers (Product # 00-5521-00) and protocol. The histogram (right) demonstrates Bcl-6 staining of CD4+CD45RO+CD278highCD279high (purple histogram) and CD4+CD45RO+CD278lowCD279low (blue histogram) cells. Cell populations were gated as indicated on the contour plot (left). Data courtesy of Danelle Eto (Laboratory of Shane Crotty).
