RQ4637

antibody from NSJ Bioreagents

Targeting: BCL6 BCL5, BCL6A, LAZ3, ZBTB27, ZNF51

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ4637

Provider

NSJ Bioreagents

Product name

Bcl6 Antibody

Antibody type

Polyclonal

Description

Antigen affinity purified

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Storage

After reconstitution, the Bcl6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) Raji, 2) MCF7, 3) placenta, 4) A549, 5) Caco-2 and 6) U-2 OS lysate with Bcl6 antibody at 0.5ug/ml. Predicted molecular weight: 78~100 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of rat 1) testis, 2) spleen, 3) brain and mouse 4) testis, 5) spleen and 6) brain lysate with Bcl6 antibody at 0.5ug/ml. Predicted molecular weight: 78~100 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE mouse spleen tissue with Bcl6 antibody at 1ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of rat BRL 3A cells with Bcl6 antibody at 1ug/10^6 cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bcl6 antibody.