Explore
Validate
Learn
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 648303 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#648303, RRID:AB_2561374
- Product name
- PE anti-mouse/human Bcl-6
- Antibody type
- Monoclonal
- Antigen
- Plasmid vector containing cDNA of the Bcl-6 gene
- Description
- B-cell lymphoma 6 (Bcl-6), is an 80 kD homodimer and member of the BTB-POZ zinc finger family. It consists of one BTB (POZ) domain and six C2H2-type zinc fingers. Bcl-6 is a transcriptional repressor as well as a master regulator of germinal center reaction. On B cells, Bcl-6 induces proliferation, antibody class switching and affinity maturation, while inhibits its differentiation to plasma cells. On T cells, Bcl-6 induces its differentiation to TFH. This molecule is also expressed in some B cell lymphomas and breast cancer cells.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 25 tests
- Storage
- The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
No comments: Submit comment
Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- Lymph node cells from a C57BL/6 mouse, at day 12, post-immunization, were surface-stained with CD19 PE/Cy7 and IgD PerCP/Cy5.5. They were then fixed and permeabilized with FOXP3 Fix/Perm buffer set and stained with Bcl-6 (clone IG191E/A8) PE. Data shown was gated on CD19+ positive cells.
- Conjugate
- Yellow dye
