Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Other assay [1]
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Validation data
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- Product number
- PA5-36753 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 46 and 54 kDa. Purity is >95% by SDS-PAGE.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-JNK1+JNK2+JNK3 pThr183/pTyr185 using Phospho-JNK1+JNK2+JNK3 pThr183/pTyr185 polyclonal antibody (Product # PA5-36753) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with UV, Lane 2: sp2/0 cell lysate treated with UV, Lane 3: PC12 cell lysate treated with UV.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) in Lane 1: MCF-7 cell lysate treated with UV, Lane 2: sp2/0 cell lysate treated with UV, Lane 3: PC12 cell lysate treated with UV. Samples were incubated with Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) polyclonal antibody (Product # PA5-36753) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: HEK293T treated with UV (15min) whole cell lysate (40 µg), Lane 3: NIH-3T3 whole cell lysate (40 µg), Lane 4: NIH-3T3 treated with UV (15min) whole cell lysate (40 µg), Lane 5: H9C2 whole cell lysate (40 µg), Lane 6: H9C2 treated with UV (4h) whole cell lysate (40 µg). Samples were incubated with Phospho-JNK1/JNK2/JNK3 (Thr183, Tyr185) polyclonal antibody (Product # PA5-36753) at a dilution of 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 10 a JNK before (CHF) and after LVAD therapy (CHF+LVAD) as compared to non-failing ventricles (NF). JNK expression was determined by immunoblot (western blot) analysis and referred to a standard, respectively, whose densitometric value was set 1 by default. The value on the y -axis, therefore, reflects the percentage of each parameter's immunoblot band density in relation to this default value. AU arbitrary unit, CHF congestive heart failure, LVAD left ventricular assist device, NF non-failing myocardial tissue specimen. b Phosphorylated JNK (pJNK) before (CHF) and after LVAD therapy (CHF+LVAD) as compared to non-failing ventricles (NF), left. The same comparisons in the subgroup of DCM patients, right ( n = 12). AU arbitrary unit, CHF congestive heart failure, DCM dilated cardiomyopathy, LVAD left ventricular assist device, NF non-failing myocardial tissue specimen