PA5-36832

antibody from Invitrogen Antibodies

Targeting: MAPK10 JNK3, p493F12, p54bSAPK, PRKM10

Antibody data

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Validation data

Product number: PA5-36832 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-JNK1/JNK2/JNK3 (Thr183) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: This antibody detects endogenous protein at a molecular weight of 46 and 54 kDa.
Concentration: 1 mg/mL

MAPK10 protein structure - PA5-36832 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-JNK1+JNK2+JNK3 pThr183 using Phospho-JNK1+JNK2+JNK3 pThr183 polyclonal antibody (Product # PA5-36832) at a dilution of 1:500. Lane 1: MCF-7 cell lysate treated with PMA, Lane 2: NIH-3T3 cell lysate treated with UV, Lane 3: PC12 cell lysate treated with PMA.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Phospho-JNK1/JNK2/JNK3 (Thr183) in Lane 1: HEK293T whole cell lysate (40 µg), Lane 2: MCF-7 whole cell lysate (40 µg), Lane 3: K562 whole cell lysate (20 µg), Lane 4: the Brain tissue lysate of rat (40 µg), Lane 5: the Kidney tissue lysate of mouse (20 µg). Samples were incubated with Phospho-JNK1/JNK2/JNK3 (Thr183) polyclonal antibody (Product # PA5-36832) at a dilution of 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-Phospho-JNK1/JNK2/JNK3 (Thr183) Polyclonal Antibody(Product # PA5-36832) and a 50kDa band corresponding to Phospho-JNK1/JNK2/JNK3 (Thr183) was observed across cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HEK-293 (Lane 2), MCF7 (Lane 3), A-431 (Lane 4), Mouse Brain (Lane 5), Rat Brain (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).