Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [4]
- Other assay [1]
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Validation data
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- Product number
- PA5-116884 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JNK1/JNK2/JNK3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Samples: SH-SY5Y, Mouse brain, Rat brain Immunogen sequence: MSRSKRDNNF YSVEIGDSTF TVLKRYQNLK PIGSGAQGIV CAAYDAILER NVAIKKLSRP FQNQTHAKRA YRELVLMKCV NHKNIIGLLN VFTPQKSLEE FQDVYIVMEL MDANLCQVIQ MELDHERMSY LLYQMLCGIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.94 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of JNK1/JNK2/JNK3 in extracts of various cell lines. Samples were incubated with JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of JNK1/JNK2/JNK3 in U2OS cells. Samples were incubated with JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of JNK1/JNK2/JNK3 in 600 μg extracts of Mouse brain cells. Samples were precipitated with 3 μg JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884). Western blot was performed from the immunoprecipitate using JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884) at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of JNK1/JNK2/JNK3 in paraffin-embedded human colon. Samples were incubated with JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of JNK1/JNK2/JNK3 in paraffin-embedded mouse brain. Samples were incubated with JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of JNK1/JNK2/JNK3 in paraffin-embedded mouse spleen. Samples were incubated with JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of JNK1/JNK2/JNK3 in paraffin-embedded rat brain. Samples were incubated with JNK1/JNK2/JNK3 Polyclonal antibody (Product # PA5-116884) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of JNK1/JNK2/JNK3 in 600 µg extracts of Mouse brain cells using JNK1/JNK2/JNK3 Polyclonal Antibody (Product # PA5-116884). Western blot was performed from the immunoprecipitate using the same antibody at a dilution of 1:100