209-401-314

antibody from Rockland Immunochemicals, Inc.

Targeting: CSF2 GM-CSF, GMCSF

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

209-401-314

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#209-401-314, RRID:AB_2085540

Product name

Anti-GM-CSF (RABBIT) Antibody - 209-401-314

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's anti-Human GM-CSF antibody shows detection of a band ~15 kDa in size corresponding to recombinant human GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG (p/n 611-143-122) secondary antibody diluted 1:20,000 in blocking buffer (p/n MB-070). Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).

Validation comment

Western Blot