MA5-35439

antibody from Invitrogen Antibodies

Targeting: COPS5 CSN5, JAB1, MOV-34, SGN5

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-35439

Provider

Invitrogen Antibodies

Product name

JAB1 Recombinant Rabbit Monoclonal Antibody (3X5F0)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAASGSGMAQ KTWELANNMQ EAQSIDEIYK YDKKQQQEIL AAKPWTKDHH YFKYCKISAL ALLKMVMHAR SGGNLEVMGL MLGKVDGETM IIMDSFALPV

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

3X5F0

Vial size

100 µL

Concentration

1.2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of JAB1 in U-2 OS cells. Samples were incubated with JAB1 Monoclonal antibody (Product # MA5-35439) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of JAB1 in NIH-3T3 cells. Samples were incubated with JAB1 Monoclonal antibody (Product # MA5-35439) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of JAB1 in paraffin-embedded human colon. Samples were incubated with JAB1 Monoclonal antibody (Product # MA5-35439) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of JAB1 in paraffin-embedded mouse testis. Samples were incubated with JAB1 Monoclonal antibody (Product # MA5-35439) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of JAB1 in paraffin-embedded rat testis. Samples were incubated with JAB1 Monoclonal antibody (Product # MA5-35439) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ChIP analysis of JAB1 in extracts of HeLa cells. Samples were incubated with JAB1 Monoclonal antibody (Product # MA5-35439) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.