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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29872 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JAB1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (99%), Xenopus laevis (99%), Pig (100%), Chicken (99%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.35 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using JAB1 Polyclonal Antibody (Product # PA5-29872). Sample (30 µg of whole cell lysate). Lane A: Hela. 10% SDS PAGE. JAB1 Polyclonal Antibody (Product # PA5-29872) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using JAB1 Polyclonal Antibody (Product # PA5-29872). Sample (50 µg of whole cell lysate). Lane A: Mouse brain. 10% SDS PAGE. JAB1 Polyclonal Antibody (Product # PA5-29872) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of JAB1 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: JAB1 Polyclonal Antibody (Product # PA5-29872) diluted at 1:200. Red: FIS1, a mitochondria marker. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- JAB1 antibody [N1C1] detects JAB1 protein at nucleus on mouse middle brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse middle brain. JAB1 antibody [N1C1] (Product # PA5-29872) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
