Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
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Validation data
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- Product number
- NBP1-86665 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86665, RRID:AB_11018045
- Product name
- Rabbit Polyclonal Migfilin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Migfilin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The interactome of LIM domain proteins: the contributions of LIM domain proteins to heart failure and heart development.
Li A, Ponten F, dos Remedios CG
Proteomics 2012 Jan;12(2):203-25
Proteomics 2012 Jan;12(2):203-25
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Migfilin Antibody [NBP1-86665] - Analysis in human cell lines A-431 and MCF-7 using Anti-FBLIM1 antibody. Corresponding FBLIM1 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Migfilin Antibody [NBP1-86665] - Analysis in control (vector only transfected HEK293T lysate) and FBLIM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665] - Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665] - Staining of human duodenum shows moderate granular cytoplasmic positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665] - Staining of human gallbladder shows moderate membranous positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665] - Staining of human kidney shows weak cytoplasmic positivity in cells in glomeruli.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Migfilin Antibody [NBP1-86665] - Staining of human skeletal muscle shows no positivity in myocytes as expected.