711634

antibody from Invitrogen Antibodies

Targeting: GRM4 GPRC1D, mGlu4, MGLUR4

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

711634

Provider

Invitrogen Antibodies

Product name

mGluR4 Recombinant Polyclonal Antibody (6HCLC)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human

Host

Rabbit

Isotype

IgG

Antibody clone number

6HCLC

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with metabotropic Glutamate Receptor 4 (mGLUR4) were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with rabbit polyclonal Anti-mGLUR4 Recombinant Rabbit Polyclonal Antibody (Product # 711634 at a concentration of 1 µg/mL). Broad Bands migrating 110 and 220 kDa corresponding to mGLUR4 monomers and dimers, respectively, were observed.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with metabotropic Glutamate Receptor 4 (mGLUR4) were lysed in detergent buffer and glycoproteins were enriched using wheat germ lectin agarose beads. Receptors were eluted from the beads using SDS sample buffer for 20 min at 45 °C. Extracts were separated on 7.5% SDS-polyacrylamide gels and blotted onto PVDF membranes. Membranes were then incubated with rabbit polyclonal Anti-mGLUR4 Recombinant Rabbit Polyclonal Antibody (Product # 711634 at a concentration of 1 µg/mL). Broad Bands migrating 110 and 220 kDa corresponding to mGLUR4 monomers and dimers, respectively, were observed.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HEK 293 cells stably transfected with metabotropic Glutamate Receptor 4 (mGLUR4) were grown on poly-L-lysine-coated coverslips overnight. After 24 h, cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times with phosphate buffer. Specimens were permeabilized with ice-cold methanol and then incubated with Anti-mGLUR4 Recombinant Rabbit Polyclonal Antibody (Product # 711634 at a concentration of 1 µg/mL). Cells were then incubated with Alexa488-conjugated secondary antibody for 2 h at room temperature, mounted and examined using a laser scanning confocal microscope. Note, strong staining of transfected cells.