A301-119A-T

antibody from Invitrogen Antibodies

Targeting: SPEN KIAA0929, MINT, RBM15C, SHARP

Western blot (Recommended by provider)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A301-119A-T

Provider

Invitrogen Antibodies

Product name

SHARP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

10 µL

Concentration

1 mg/mL

Storage

4° C

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SHARP by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHARP antibody (Product # A301-119A; Lot 3) used for IP at 6 µg per reaction. SHARP was also immunoprecipitated by a second antibody against a different epitope of SHARP (Product # A301-120A). For blotting immunoprecipitated SHARP, Product # A301-119A was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human SHARP by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SHARP antibody A301-119A (lot A301-119A-2) used for IP at 6 µg per reaction. For blotting immunoprecipitated SHARP, A301-119A was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.